5' copyback defective viral genomes are major component in clinical and non-clinical influenza samples.

ABSTRACT: Clinical samples from people with influenza disease have been analyzed to assess the presence and abundance of Defective Viral Genomes (DVGs), but these have not been assessed using the same bioinformatic pipeline. The type of DVG most described for influenza infections (deletion DVGs) differs from the most commonly described DVGs from non-segmented negative stranded viruses (5' copyback). This could be attributed to either differences between viruses or the tools used to detect and characterize DVGs. Here we analyze several NGS datasets from people infected with different types of influenza virus using the same bioinformatic pipeline. We observe that 5' copyback DVGs are prevalent in all human clinical samples but not in the cultured samples. To address this discrepancy between clinical a

SUBMITTER: Li X 

PROVIDER: S-EPMC10701078 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature