Establishment and application of a VP3 antigenic domain-based peptide ELISA for the detection of antibody against goose plague virus infection.

ABSTRACT: The detection of antibody against goose plague virus (GPV) infection has never had a commercialized test kit, which has posed challenges to the prevention and control of this disease. In this study, bioinformatics software was used to analyze and predict the dominant antigenic regions of the main protective antigen VP3 of GPV. Three segments of bovine serum albumin (BSA) vector-coupled peptides were synthesized as ELISA coating antigens. Experimental results showed that the VP3-1 (358-392aa) peptide had the best reactivity and specificity. By using the BSA-VP3-1 peptide, a detection method for antibody against GPV infection was established, demonstrating excellent specificity with no cross-reactivity with common infectious goose pathogen antibodies. The intra-batch coefficient of variation

SUBMITTER: Qiao D 

PROVIDER: S-EPMC10701384 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature