Protocol to image and analyze hippocampal network dynamics in non-anesthetized mouse pups.

ABSTRACT: Two-photon calcium imaging is a powerful technique that has revolutionized our understanding of how neural circuit dynamics supports different behaviors and cognitive processes. However, performing imaging during development remains challenging. Here, we provide a protocol to image CA1 neurons in mouse pups as well as a pipeline of analysis to analyze and share the data. We describe steps for intracerebroventricular injection, cranial window surgery, two-photon calcium imaging, and analysis of imaging data. For complete details on the use and execution of this protocol, please refer to Dard et al.¹ and Denis et al.².

SUBMITTER: Ratsifandrihamanana MR 

PROVIDER: S-EPMC10701450 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature