Mutation of self-binding sites in the promoter of the MrpC transcriptional regulator leads to asynchronous Myxococcus xanthus development.

ABSTRACT:

Introduction

MrpC, a member of the CRP/Fnr transcription factor superfamily, is necessary to induce and control the multicellular developmental program of the bacterium, Myxococcus xanthus. During development, certain cells in the population first swarm into haystack-shaped aggregates and then differentiate into environmentally resistant spores to form mature fruiting bodies (a specialized biofilm). mrpC transcriptional regulation is controlled by negative autoregulation (NAR).

Methods

Wild type and mutant mrpC promoter regions were fused to a fluorescent reporter to examine effects on mrpC expression in the population and in single cells in situ. Phenotypic consequences of the mutant mrpC promoter were assayed by deep convolution ne

SUBMITTER: McLaughlin M 

PROVIDER: S-EPMC10701913 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature