Biochemical and genetic studies define the functions of methylthiotransferases in methanogenic and methanotrophic archaea.

ABSTRACT: Methylthiotransferases (MTTases) are radical S-adenosylmethionine (SAM) enzymes that catalyze the addition of a methylthio (-SCH₃) group to an unreactive carbon center. These enzymes are responsible for the production of 2-methylthioadenosine (ms²A) derivatives found at position A37 of select tRNAs in all domains of life. Additionally, some bacteria contain the RimO MTTase that catalyzes the methylthiolation of the S12 ribosomal protein. Although the functions of MTTases in bacteria and eukaryotes have been established via detailed genetic and biochemical studies, MTTases from the archaeal domain of life are understudied and the substrate specificity determinants of MTTases remain unclear. Here, we report the in vitro enzymatic activities of an MTTase (C

SUBMITTER: Boswinkle K 

PROVIDER: S-EPMC10702137 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature