Automated pipeline for nerve fiber selection and g-ratio calculation in optical microscopy: exploring staining protocol variations.

ABSTRACT: G-ratio is crucial for understanding the nervous system's health and function as it measures the relative myelin thickness around an axon. However, manual measurement is biased and variable, emphasizing the need for an automated and standardized technique. Although deep learning holds promise, current implementations lack clinical relevance and generalizability. This study aimed to develop an automated pipeline for selecting nerve fibers and calculating relevant g-ratio using quality parameters in optical microscopy. Histological sections from the sciatic nerves of 16 female mice were prepared and stained with either p-phenylenediamine (PPD) or toluidine blue (TB). A custom UNet model was trained on a mix of both types of staining to segment the sections based on 7,694 manually delineated

SUBMITTER: Thomson BR 

PROVIDER: S-EPMC10702553 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature