Enhancement of mucosal innate and adaptive immunity following intranasal immunization of mice with a bovine adenoviral vector.

ABSTRACT:

Introduction

Nonhuman adenoviral (AdV) gene delivery platforms have significant value due to their ability to elude preexisting AdV vector immunity in most individuals. Previously, we have demonstrated that intranasal (IN) immunization of mice with BAd-H5HA, a bovine AdV type 3 (BAdV3) vector expressing H5N1 influenza virus hemagglutinin (HA), resulted in enhanced humoral and cell-mediated immune responses. The BAd-H5HA IN immunization resulted in complete protection following the challenge with an antigenically distinct H5N1 virus compared to the mouse group similarly immunized with HAd-H5HA, a human AdV type 5 (HAdV5) vector expressing HA.

Methods

Here, we attempted to determine the activation of innate immune responses in the lungs of mice inoculated intranasally with BAd

SUBMITTER: Sayedahmed EE 

PROVIDER: S-EPMC10702558 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature