The transcriptomics profiling of blood CD4 and CD8 T-cells in narcolepsy type I.

ABSTRACT:

Background

Narcolepsy Type I (NT1) is a rare, life-long sleep disorder arising as a consequence of the extensive destruction of orexin-producing hypothalamic neurons. The mechanisms involved in the destruction of orexin neurons are not yet elucidated but the association of narcolepsy with environmental triggers and genetic susceptibility (strong association with the HLA, TCRs and other immunologically-relevant loci) implicates an immuno-pathological process. Several studies in animal models and on human samples have suggested that T-cells are the main pathogenic culprits.

Methods

RNA sequencing was performed on four CD4 and CD8 T-cell subsets (naive, effector, effector memory and central memory) sorted by flow cytometry from peripheral blood mononuclear cells (PBMCs) of NT1

SUBMITTER: Khajavi L 

PROVIDER: S-EPMC10702585 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature