Combining organotypic tissue culture with light-sheet microscopy (OTCxLSFM) to study glioma invasion.

ABSTRACT: Glioblastoma is a very aggressive tumor and represents the most common primary brain malignancy. Key characteristics include its high resistance against conventional treatments, such as radio- and chemotherapy and its diffuse tissue infiltration, preventing complete surgical resection. The analysis of migration and invasion processes in a physiological microenvironment allows for enhanced understanding of these phenomena and can lead to improved therapeutic approaches. Here, we combine two state-of-the-art techniques, adult organotypic brain tissue slice culture (OTC) and light-sheet fluorescence microscopy (LSFM) of cleared tissues in a combined method termed OTCxLSFM. Using this methodology, we can show that glioblastoma tissue infiltration can be effectively blocked through treatment wi

SUBMITTER: Haydo A 

PROVIDER: S-EPMC10702825 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature