Single-cell transcriptomics reveals peripheral immune responses in non-segmental vitiligo.

ABSTRACT:

Background

Vitiligo is a common autoimmune depigmented dermatology due to destruction of melanocytes. Much evidence suggests that vitiligo is associated with systemic immune activation. Previous studies have focused on immune cell infiltration in and around lesion areas, but few studies have investigated the cell types and function of circulating immune cells in peripheral blood. Here, single cell RNA-sequencing (scRNA-seq) was used to investigate the mechanisms of peripheral immune responses in vitiligo patients.

Methods

Peripheral blood was collected from five patients with progressive non-segmental vitiligo and three healthy controls. Peripheral blood mononuclear cells (PBMCs) were obtained by Ficoll-Paque density gradient centrifugation, and scRNA-seq was performed on is

SUBMITTER: Yang P 

PROVIDER: S-EPMC10702986 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature