Methods for intratumoral microdialysis probe targeting and validation in murine brain tumor models.

ABSTRACT:

Background

Microdialysis is a well validated sampling technique that can be used for pharmacokinetic studies of oncological drugs targeting the central nervous system. This technique has also been applied to evaluate tumor metabolism and identify pharmacodynamic biomarkers of drug activity. Despite the potential utility of microdialysis for therapeutic discovery, variability in tumor size and location hamper routine use of microdialysis as a preclinical tool. Quantitative validation of microdialysis membrane location relative to radiographically evident tumor regions could facilitate rigorous preclinical studies. However, a widely accessible standardized workflow for preclinical catheter placement and validation is needed.

New method

We provide methods for a workflow to yiel

SUBMITTER: Rajani K 

PROVIDER: S-EPMC10703144 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature