Escherichia coli adhesion protein FimH exacerbates colitis via CD11b⁺CD103^- dendritic cell activation.

ABSTRACT:

Introduction

Immune stimulators are used to improve vaccine efficiency; however, they are accompanied by various side effects. In previous studies, we reported that the Escherichia coli adhesion protein, FimH, induces immune activity; however, we did not examine any side effects in colon inflammation.

Methods

FimH was administered orally or intraperitoneally (i.p.) to mice with dextran sulfate sodium (DSS)-induced colitis, and changes in symptoms were observed. Immune cells infiltrated into the colon after the induction of colon inflammation were analyzed using a flow cytometer. Changes in Th1 and Th17 cells that induce colitis were analyzed. Further, mesenteric lymph node (mLN) dendritic cells (DCs) activated by FimH were identified and isolated to examine the

SUBMITTER: Zhang W 

PROVIDER: S-EPMC10703180 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature