First insight into extracellular vesicle-miRNA characterization in a sheep in vitro model of inflammation.

ABSTRACT: Extracellular vesicles (EVs) and their microRNA (miRNA) cargoes have garnered attention in the veterinary field for their regulatory role in various biological processes. This study aimed to (i) evaluate two techniques of EV isolation from sheep peripheral blood mononuclear cell (PBMC) supernatants using the ultracentrifugation (UC) and reagent (REA) methods and (ii) characterize the EV-miRNA profiles after an in vitro inflammatory environment mediated by lipopolysaccharides (LPS). Sheep peripheral blood was collected, and PBMCs were separated using a density gradient reagent. Subsequently, PBMCs were cultured at 37°C for 24 h (5% CO₂), and the supernatants were collected to perform the EV isolation. The presence of CD81⁺ extracellular vesicle marker was determ

SUBMITTER: Ciliberti MG 

PROVIDER: S-EPMC10703394 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature