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ABSTRACT: Purpose
This study aimed to investigate the potential role of copine-1 (CPNE1), a calcium-dependent membrane-binding protein encoded by the CPNE1 gene, in colorectal cancer (CRC). Despite previous research on the involvement of copine family members in various solid tumors, the specific role of CPNE1 in CRC remains poorly understood.Methods
We conducted clinicopathological analysis and functional studies to explore the impact of CPNE1 in human CRC. We examined the expression levels of CPNE1 in CRC patients and correlated it with invasive depth, lymph node metastasis, distant metastasis, lymphatic invasion, and TNM stage. Additionally, we performed experiments to assess the functional consequences of CPNE1 knockdown in CRC cells, including proliferation, colony formation, migration, invasion, and the expression of key regulators involved in the cell cycle and epithelial-mesenchymal transition (EMT). Furthermore, we evaluated the effects of CPNE1 knockdown on tumor growth using a xenograft mouse model.Results
High expression of CPNE1 was significantly associated with advanced tumor features in CRC patients. CPNE1 knockdown in CRC cells led to impaired abilities in proliferation, colony formation, migration, and invasion. Furthermore, CPNE1 silencing resulted in the suppression of protein expression related to the cell cycle and EMT. In the xenograft mouse model, CPNE1 knockdown inhibited tumor growth.Conclusion
CPNE1 plays a crucial role in promoting tumorigenesis and metastasis in human CRC. By regulating the cell cycle and EMT, CPNE1 influences critical cellular processes at the membrane-cytoplasm interface. These results provide valuable insights into the potential development of novel therapeutic strategies for CRC targeting CPNE1.
SUBMITTER: Lee JK
PROVIDER: S-EPMC10703583 | biostudies-literature | 2023 Dec
REPOSITORIES: biostudies-literature
Lee Jin-Kwon JK Lee Seung-Jun SJ Hah Young-Sool YS Ji Yeong-Ho YH Ju Young-Tae YT Lee Young-Joon YJ Jeong Chi-Young CY Kim Ju-Yeon JY Park Ji-Ho JH Kim Jae-Myung JM Cho Jin-Kyu JK Kim Han-Gil HG Kwag Seung-Jin SJ
Annals of surgical treatment and research 20231129 6
<h4>Purpose</h4>This study aimed to investigate the potential role of copine-1 (CPNE1), a calcium-dependent membrane-binding protein encoded by the <i>CPNE1</i> gene, in colorectal cancer (CRC). Despite previous research on the involvement of copine family members in various solid tumors, the specific role of CPNE1 in CRC remains poorly understood.<h4>Methods</h4>We conducted clinicopathological analysis and functional studies to explore the impact of CPNE1 in human CRC. We examined the expressi ...[more]