An FNR-type regulator controls the anaerobic expression of hyn hydrogenase in Thiocapsa roseopersicina.

ABSTRACT: The purple sulfur photosynthetic bacterium Thiocapsa roseopersicina BBS contains a heat-stable membrane-associated hydrogenase encoded by the hyn operon. Expression from the hyn operon regulatory region is up-regulated under anaerobic conditions. cis elements were mapped between positions -602 and -514 upstream from the hynS gene. Within this region two sequences that resemble DNA sites for FNR were recognized. The gene of an FNR homologue, FnrT, was identified in the genome of T. roseopersicina, and an fnrT knockout mutant was constructed. Anaerobic induction of hynS expression was abolished in the fnrT mutant, suggesting that FnrT is an activator of the hynS promoter. The T. roseopersicina hynS promoter could be activated in Escherichia coli, and this regulation was dependent on E. coli

SUBMITTER: Kovacs AT 

PROVIDER: S-EPMC1070371 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature