Multiplane HiLo microscopy with speckle illumination and non-local means denoising.

ABSTRACT:

Significance

HiLo microscopy synthesizes an optically sectioned image from two images, one obtained with uniform and another with patterned illumination, such as laser speckle. Speckle-based HiLo has the advantage of being robust to aberrations but is susceptible to residual speckle noise that is difficult to control. We present a computational method to reduce this residual noise without undermining resolution. In addition, we improve the versatility of HiLo microscopy by enabling simultaneous multiplane imaging (here nine planes).

Aim

Our goal is to perform fast, high-contrast, multiplane imaging with a conventional camera-based fluorescence microscope.

Approach

Multiplane HiLo imaging is achieved with the use of a single camera and z-splitter prism. Speckle noise r

SUBMITTER: Zheng S 

PROVIDER: S-EPMC10704089 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature