Comparison of consecutive and restained sections for image registration in histopathology.

ABSTRACT:

Significance

Although the registration of restained sections allows nucleus-level alignment that enables a direct analysis of interacting biomarkers, consecutive sections only allow the transfer of region-level annotations. The latter can be achieved at low computational cost using coarser image resolutions.

Purpose

In digital histopathology, virtual multistaining is important for diagnosis and biomarker research. Additionally, it provides accurate ground truth for various deep-learning tasks. Virtual multistaining can be obtained using different stains for consecutive sections or by restaining the same section. Both approaches require image registration to compensate for tissue deformations, but little attention has been devoted to comparing their accuracy.

Approach

SUBMITTER: Lotz J 

PROVIDER: S-EPMC10704256 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature