Selective and sensitive fluorescent staining of serum albumin in protein gel electrophoresis via sequence-defined oligo-dithiocarbamate.

ABSTRACT: This work presents a fluorescent sequence-defined oligo dithiocarbamate platform with a dansyl appendage for interaction studies with a range of proteins including BSA, HSA, proteinase, trypsin, lysozyme, hemoglobin, and amylase. The platform involves six distinct sequence-defined oligomers (SDOs), each offering varied functionalities - dithiocarbamate (DTC), ester, and amide - within the backbone and side chains; different architectures (linear and branched); and introduction of polar or non-polar groups. Fluorescence titration experiments and molecular docking were used to explore the interaction between the synthesized SDOs and the listed proteins. This analysis identified two promising candidates, particularly SDOs 1 and 2, based on higher FRET efficiency, indicating a stronger interac

SUBMITTER: Jose A 

PROVIDER: S-EPMC10704300 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature