Simple Tool for Rapidly Assessing the Quality of Multiplexed Single Cell Proteomics Data.

ABSTRACT: Recent advances in the sensitivity and speed of mass spectrometers coupled with improved sample preparation methods have enabled the field of single cell proteomics to proliferate. While heavy development is occurring in the label free space, dramatic improvements in throughput are provided by multiplexing with tandem mass tags. Hundreds or thousands of single cells can be analyzed with this method, yielding large data sets which may contain poor data arising from loss of material during cell sorting or poor digestion, labeling, and lysis. To date, no tools have been described that can assess data quality prior to data processing. We present herein a lightweight python script and accompanying graphic user interface that can rapidly quantify reporter ion peaks within each MS/MS spectrum in

SUBMITTER: Jenkins C 

PROVIDER: S-EPMC10704589 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature