Investigation of nanotopography on SOCE mediated cell migration via live-cell : Imaging on opaque implant surface.

ABSTRACT: The exploration of cell response to nanotopography has attracted considerable attentions for years. This article focuses on the influence of nanotopography on the intracellular Ca²⁺ dynamics, the most ubiquitous but ignored second messenger. The classic titanium nanotubes (NT) were fabricated by anodization to formulate nanoporous surfaces. Firstly, the store operative calcium entry (SOCE) in endoplasmic reticulum (ER) and functional Ca²⁺ release-activated Ca²⁺ (CRAC) channels were significantly enhanced on NT surfaces that revealed by live-cell Ca²⁺ imaging and fluorescence resonance energy transfer (FRET) identification of orai1-stim1 connection. To investigate the potential implication of Ca²⁺ elevation, the dynamic cell migration t

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10704632 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature