Multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancer.

ABSTRACT:

Background

Breast cancer (BC) patients tend to suffer from distant metastasis, especially bone metastasis.

Methods

All the analysis based on open-accessed data was performed in R software, dependent on multiple algorithms and packages. The RNA levels of specific genes were detected using quantitative Real-time PCR as a method of detecting the RNA levels. To assess the ability of BC cells to proliferate, we utilized the CCK8 test, colony formation, and the 5-Ethynyl-20-deoxyuridine assay. BC cells were evaluated for invasion and migration by using Transwell assays and wound healing assays.

Results

In our study, we identified the molecules involved in BC bone metastasis based on the data from multiple BC cohorts. Then, we comprehensively investigated the effect pattern

SUBMITTER: Yao K 

PROVIDER: S-EPMC10704724 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature