Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids.

ABSTRACT: The intestinal epithelium plays an essential role in human health, providing a barrier between the host and the external environment. This highly dynamic cell layer provides the first line of defense between microbial and immune populations and helps to modulate the intestinal immune response. Disruption of the epithelial barrier is a hallmark of inflammatory bowel disease (IBD) and is of interest for therapeutic targeting. The 3-dimensional colonoid culture system is an extremely useful in vitro model for studying intestinal stem cell dynamics and epithelial cell physiology in IBD pathogenesis. Ideally, establishing colonoids from the inflamed epithelial tissue of animals would be most beneficial in assessing the genetic and molecular influences on disease. However, we have shown that in

SUBMITTER: Crawford E 

PROVIDER: S-EPMC10705019 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature