An Optimized Method to Culture Human Primary Lung Tumor Cell Spheroids.

ABSTRACT: Lung cancer is the leading cause of cancer mortality worldwide, with a median survival rate at 5 years of less than 20%. While molecular mapping aids in selecting appropriate therapies, it cannot predict personalized treatment response and long-term efficacy. For addressing these challenges, there is a great need for functional tests. Within this context, we developed patient-derived spheroids (PDS) from tumor and adjacent normal tissue to biomimic the respective tissue for assessing the personalized drug treatment response in vitro. Surgically resected lung specimens were used to generate spheroids using a two-step culture procedure. Flow cytometry and immune staining enabled the characterization of different cell populations resulting from the lung samples. PDS phenotype, cell proliferat

SUBMITTER: Mueggler A 

PROVIDER: S-EPMC10705303 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature