Engineered <i>Bacillus subtilis</i> as oral probiotics to enhance clearance of blood lactate.

ABSTRACT: Elevated lactate concentrations are implicated in various acute and chronic diseases such as sepsis and mitochondrial dysfunction, respectively. Conversely, ineffective lactate clearance is associated with poor clinical prognoses and high mortality in these diseases. While several groups have proposed using small molecule inhibitors and enzyme replacement to reduce circulating lactate, there are few practical and effective ways to manage this condition. Recent evidence suggests that lactate is exchanged between systemic circulation and the gut, allowing bidirectional modulation between the gut microbiota and peripheral tissues. Inspired by these findings, this work seeks to engineer spore-forming probiotic B. subtilis strains to enable intestinal delivery of lactate oxidase as a the

SUBMITTER: Yang M 

PROVIDER: S-EPMC10705430 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature