Profiling Expression Strategies for a Type III Polyketide Synthase in a Lysate-Based, Cell-free System.

ABSTRACT: Some of the most metabolically diverse species of bacteria (e.g., Actinobacteria) have higher GC content in their DNA, differ substantially in codon usage, and have distinct protein folding environments compared to tractable expression hosts like Escherichia coli. Consequentially, expressing biosynthetic gene clusters (BGCs) from these bacteria in E. coli frequently results in a myriad of unpredictable issues with protein expression and folding, delaying the biochemical characterization of new natural products. Current strategies to achieve soluble, active expression of these enzymes in tractable hosts, such as BGC refactoring, can be a lengthy trial-and-error process. Cell-free expression (CFE) has emerged as 1) a valuable expression platform for enzymes that are challenging

SUBMITTER: Sword TT 

PROVIDER: S-EPMC10705458 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature