CopepodTCR: Identification of Antigen-Specific T Cell Receptors with combinatorial peptide pooling.

ABSTRACT: T cell receptor (TCR) repertoire diversity enables the orchestration of antigen-specific immune responses against the vast space of possible pathogens. Identifying TCR/antigen binding pairs from the large TCR repertoire and antigen space is crucial for biomedical research. Here, we introduce copepodTCR, an open-access tool for the design and interpretation of high-throughput experimental assays to determine TCR specificity. copepodTCR implements a combinatorial peptide pooling scheme for efficient experimental testing of T cell responses against large overlapping peptide libraries, useful for "deorphaning" TCRs of unknown specificity. The scheme detects experimental errors and, coupled with a hierarchical Bayesian model for unbiased results interpretation, identifies the resp

SUBMITTER: Kovaleva VA 

PROVIDER: S-EPMC10705480 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature