YAP localization mediates mechanical adaptation of human cancer cells during extravasation in vivo.

ABSTRACT: Biophysical profiling of primary tumors has revealed that individual tumor cells fall along a highly heterogeneous continuum of mechanical phenotypes. One idea is that a subset of tumor cells is "softer" to facilitate detachment and escape from the primary site, a step required to initiate metastasis. However, it has also been postulated that cells must be able to deform and generate sufficient force to exit into distant sites. Here, we aimed to dissect the mechanical changes that occur during extravasation and organ colonization. Using multiplexed methods of intravital microscopy and optical tweezer based active microrheology, we obtained longitudinal images and mechanical profiles of cells during organ colonization in vivo. We determined that cells were softer, more liquid like up

SUBMITTER: So WY 

PROVIDER: S-EPMC10705547 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature