In situ lipidomics of Staphylococcus aureus osteomyelitis using imaging mass spectrometry.

ABSTRACT: Osteomyelitis occurs when Staphylococcus aureus invades the bone microenvironment, resulting in a bone marrow abscess with a spatially defined architecture of cells and biomolecules. Imaging mass spectrometry and microscopy are invaluable tools that can be employed to interrogate the lipidome of S. aureus-infected murine femurs to reveal metabolic and signaling consequences of infection. Here, nearly 250 lipids were spatially mapped to healthy and infection-associated morphological features throughout the femur, establishing composition profiles for tissue types. Ether lipids and arachidonoyl lipids were significantly altered between cells and tissue structures in abscesses, suggesting their roles in abscess formation and inflammatory signaling. Sterols, triglycerides, bis(mo

SUBMITTER: Good CJ 

PROVIDER: S-EPMC10705574 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature