Astroglial S100B Secretion Is Mediated by Ca²⁺ Mobilization from Endoplasmic Reticulum: A Study Using Forskolin and DMSO as Secretagogues.

ABSTRACT: S100B, a homodimeric Ca²⁺-binding protein, is produced and secreted by astrocytes, and its extracellular levels have been used as a glial marker in brain damage and neurodegenerative and psychiatric diseases; however, its mechanism of secretion is elusive. We used primary astrocyte cultures and calcium measurements from real-time fluorescence microscopy to investigate the role of intracellular calcium in S100B secretion. In addition, the dimethyl sulfoxide (DMSO) effect on S100B was investigated in vitro and in vivo using Wistar rats. We found that DMSO, a widely used vehicle in biological assays, is a powerful S100B secretagogue, which caused a biphasic response of Ca²⁺ mobilization. Our data show that astroglial S100B secretion is triggered by the increase in intrac

SUBMITTER: Leite MC 

PROVIDER: S-EPMC10706453 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature