Influence of Organ Culture on the Characteristics of the Human Limbal Stem Cell Niche.

ABSTRACT: Organ culture storage techniques for corneoscleral limbal (CSL) tissue have improved the quality of corneas for transplantation and allow for longer storage times. Cultured limbal tissue has been used for stem cell transplantation to treat limbal stem cell deficiency (LSCD) as well as for research purposes to assess homeostasis mechanisms in the limbal stem cell niche. However, the effects of organ culture storage conditions on the quality of limbal niche components are less well described. Therefore, in this study, the morphological and immunohistochemical characteristics of organ-cultured limbal tissue are investigated and compared to fresh limbal tissues by means of light and electron microscopy. Organ-cultured limbal tissues showed signs of deterioration, such as edema, less pronounced

SUBMITTER: Polisetti N 

PROVIDER: S-EPMC10706739 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature