Time-Resolved X-ray Observation of Intracellular Crystallized Protein in Living Animal.

ABSTRACT: Understanding the cellular environment as molecular crowding that supports the structure-specific functional expression of biomolecules has recently attracted much attention. Time-resolved X-ray observations have the remarkable capability to capture the structural dynamics of biomolecules with subnanometre precision. Nevertheless, the measurement of the intracellular dynamics within live organisms remains a challenge. Here, we explore the potential of utilizing crystallized proteins that spontaneously form intracellular crystals to investigate their intracellular dynamics via time-resolved X-ray observations. We generated transgenic Caenorhabditis elegans specifically expressing the crystallized protein in cells and observed the formation of the protein aggregates within the animal

SUBMITTER: Kuramochi M 

PROVIDER: S-EPMC10706802 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature