Spatial Transcriptomics Reveals Signatures of Histopathological Changes in Muscular Sarcoidosis.

ABSTRACT: Sarcoidosis is a multisystemic disease characterized by non-caseating granuloma infiltrating various organs. The form with symptomatic muscular involvement is called muscular sarcoidosis. The impact of immune cells composing the granuloma on the skeletal muscle is misunderstood. Here, we investigated the granuloma-skeletal muscle interactions through spatial transcriptomics on two patients affected by muscular sarcoidosis. Five major transcriptomic clusters corresponding to perigranuloma, granuloma, and three successive muscle tissue areas (proximal, intermediate, and distal) around the granuloma were identified. Analyses revealed upregulated pathways in the granuloma corresponding to the activation of T-lymphocytes and monocytes/macrophages cytokines, the upregulation of extracellular mat

SUBMITTER: Lequain H 

PROVIDER: S-EPMC10706822 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature