Radiobiological Assessment of Targeted Radionuclide Therapy with [¹⁷⁷Lu]Lu-PSMA-I&T in 2D vs. 3D Cell Culture Models.

ABSTRACT: In vitro therapeutic efficacy studies are commonly conducted in cell monolayers. However, three-dimensional (3D) tumor spheroids are known to better represent in vivo tumors. This study used [¹⁷⁷Lu]Lu-PSMA-I&T, an already clinically applied radiopharmaceutical for targeted radionuclide therapy against metastatic castrate-resistant prostate cancer, to demonstrate the differences in the radiobiological response between 2D and 3D cell culture models of the prostate cancer cell lines PC-3 (PSMA negative) and LNCaP (PSMA positive). After assessing the target expression in both models via Western Blot, cell viability, reproductive ability, and growth inhibition were assessed. To investigate the geometric effects on dosimetry for the 2D vs. 3D models, Monte Carlo simulations were perfo

SUBMITTER: Raitanen J 

PROVIDER: S-EPMC10706939 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature