Transcriptional Targeting of Dendritic Cells Using an Optimized Human Fascin1 Gene Promoter.

ABSTRACT: Deeper knowledge about the role of the tumor microenvironment (TME) in cancer development and progression has resulted in new strategies such as gene-based cancer immunotherapy. Whereas some approaches focus on the expression of tumoricidal genes within the TME, DNA-based vaccines are intended to be expressed in antigen-presenting cells (e.g., dendritic cells, DCs) in secondary lymphoid organs, which in turn induce anti-tumor T cell responses. Besides effective delivery systems and the requirement of appropriate adjuvants, DNA vaccines themselves need to be optimized regarding efficacy and selectivity. In this work, the concept of DC-focused transcriptional targeting was tested by applying a plasmid encoding for the luciferase reporter gene under the control of a derivative of the human

SUBMITTER: Zeyn Y 

PROVIDER: S-EPMC10706967 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature