Ambroxol-Enhanced Frequency and Amplitude of Beating Cilia Controlled by a Voltage-Gated Ca²⁺ Channel, Cav1.2, via pH_i Increase and [Cl^-]_i Decrease in the Lung Airway Epithelial Cells of Mice.

ABSTRACT: Ambroxol (ABX), a frequently prescribed secretolytic agent which enhances the ciliary beat frequency (CBF) and ciliary bend angle (CBA, an index of amplitude) by 30%, activates a voltage-dependent Ca²⁺ channel (Ca_V1.2) and a small transient Ca²⁺ release in the ciliated lung airway epithelial cells (c-LAECs) of mice. The activation of Ca_V1.2 alone enhanced the CBF and CBA by 20%, mediated by a pH_i increase_i and a [Cl^-]_i decrease in the c-LAECs. The increase in pH_i, which was induced by the activation of the Na⁺-HCO₃^- cotransporter (NBC), enhanced the CBF (by 30%) and CBA (by 15-20%), and a decrease in [Cl^-]_i, which was induced by the Cl

SUBMITTER: Nakahari T 

PROVIDER: S-EPMC10707002 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature