Second Wave, Late-Stage Neuroinflammation in Cleared Brains of Aged 5xFAD Alzheimer's Mice Detected by Macrolaser Light Sheet Microscopy Imaging.

ABSTRACT: This study leverages the innovative imaging capabilities of macrolaser light-sheet microscopy to elucidate the 3D spatial visualization of AD-associated neuropathologic networks in the transparent brains of 44-week-old 5xFAD mice. Brain samples from ten AD and seven control mice were prepared through a hydrophilic tissue-clearing pipeline and immunostained with thioflavin S (β-amyloid), anti-CD11b antibody (microglia), and anti-ACSA-2 antibody (astrocytes). The 5xFAD group exhibited significantly higher average total surface volumes of β-amyloid accumulation than the control group (AD, 898,634,368 µm³ [383,355,488-1,324,986,752]; control, 33,320,178 µm³ [11,156,785-65,390,988], p = 0.0006). Within the AD group, there was significant interindividual and interind

SUBMITTER: Lee SH 

PROVIDER: S-EPMC10707588 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature