Rapid detection of Mycobacterium tuberculosis using recombinase polymerase amplification: A pilot study.

ABSTRACT: Tuberculosis remains one of the leading causes of death worldwide, especially in low- and middle-income countries. Tuberculosis treatment and control efforts are hindered by the difficulty in making the diagnosis, as currently available diagnostic tests are too slow, too expensive, or not sufficiently sensitive. Recombinase polymerase amplification (RPA) is a novel technique that allows for the amplification of DNA rapidly, at constant temperature, and with minimal expense. We calculated and compared the limit of detection, sensitivity, and specificity of two RPA-based assays for the diagnosis of pulmonary tuberculosis, using two sets of published primers. We also calculated and compared the assays' limits of detection and compared their performance using two different DNA extraction metho

SUBMITTER: Sciaudone M 

PROVIDER: S-EPMC10707601 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature