Development of Stable CHO-K1 Cell Lines Overexpressing Full-Length Human CD20 Antigen.

ABSTRACT:

Background

CD20 is a differentiation-related antigen exclusively expressed on the membrane of B lymphocytes. CD20 amplification is observed in numerous immune-related disorders, making it an ideal target for immunotherapy of hematological malignancies and autoimmune diseases. MAb-based therapies targeting CD20 have a principal role in the treatment of several immune-related disordes and cancers, including CLL. Fc gamma receptors mediate CD20 internalization in hematopoietic cells; therefore, this study aimed to establish non-hematopoietic stable cell lines overexpressing full-length human CD20 antigen as an in vitro model for CD20-related studies.

Methods

CD20 gene was cloned into the transfer vector. The lentivirus system was transfected to packaging HEK 293T cells, and the

SUBMITTER: Mohammadkhani N 

PROVIDER: S-EPMC10707815 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature