Resistomycin Suppresses Prostate Cancer Cell Growth by Instigating Oxidative Stress, Mitochondrial Apoptosis, and Cell Cycle Arrest.

ABSTRACT: Globally, prostate cancer is among the most threatening and leading causes of death in men. This study, therefore, aimed to search for an ideal antitumor strategy with high efficacy, low drug resistance, and no or few adverse effects. Resistomycin is a natural antibiotic derived from marine actinomycetes, and it possesses various biological activities. Prostate cancer cells (PC3) were treated with resistomycin (IC₁₂.₅: 0.65 or IC₂₅: 1.3 µg/mL) or 5-fluorouracil (5-FU; IC₂₅: 7 µg/mL) for 24 h. MTT assay and flow cytometry were utilized to assess cell viability and apoptosis. Oxidative stress, apoptotic-related markers, and cell cycle were also assessed. The results revealed that the IC₅₀ of resistomycin and 5-FU on PC3 cells were 2.63 µ

SUBMITTER: Aloufi AS 

PROVIDER: S-EPMC10708360 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature