Regenerating human skeletal muscle forms an emerging niche in vivo to support PAX7 cells.

ABSTRACT: Skeletal muscle stem and progenitor cells including those derived from human pluripotent stem cells (hPSCs) offer an avenue towards personalized therapies and readily fuse to form human-mouse myofibres in vivo. However, skeletal muscle progenitor cells (SMPCs) inefficiently colonize chimeric stem cell niches and instead associate with human myofibres resembling foetal niches. We hypothesized competition with mouse satellite cells (SCs) prevented SMPC engraftment into the SC niche and thus generated an SC ablation mouse compatible with human engraftment. Single-nucleus RNA sequencing of SC-ablated mice identified the absence of a transient myofibre subtype during regeneration expressing Actc1. Similarly, ACTC1⁺ human myofibres supporting PAX7⁺ SMPCs increased in SC-abl

SUBMITTER: Hicks MR 

PROVIDER: S-EPMC10709143 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature