LncRNA-HANR exacerbates malignant behaviors of cholangiocarcinoma cells through activating Notch pathway.

ABSTRACT:

Objectives

Cholangiocarcinoma (CHOL) is a malignant tumor from extrahepatic bile duct with poor prognosis. The critical roles of long non-coding RNAs (lncRNAs) in cancers including CHOL have been unveiled in recent decades. The present study was aimed to investigate the role and mechanism of a certain lncRNA, namely, hepatocellular carcinoma (HCC) associated long non-coding RNA (HANR) in CHOL.

Methods

Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was applied for detecting gene expression. Functional assays were done for assessing CHOL cell malignancy and mechanistic assays were conducted for analyzing correlation between HANR and Notch signal pathway, as well as the relation between HANR and Notch intracellular domain (NICD) in CHOL cells.

Results

SUBMITTER: Zhou G 

PROVIDER: S-EPMC10709194 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature