Next Generation Sequencing Outperforms Cultivation-Based Methods for Detection of Bacterial Genera in Bile After Liver Transplantation.

ABSTRACT:

Background and aims

Bacterial cholangitis is a common complication in patients with ischemic type biliary lesions and/or anastomotic strictures after liver transplantation (LTX). Patients frequently need antibiotics and endoscopic retrograde cholangiography (ERC) to improve the bile flow. Antibiotic treatment is based on findings in standard microbiological cultivation (SMC) of bile. However, the cultivation techniques are limited to a subset of bacteria easy-to-cultivate. Therefore, the aim of our study was to evaluate the value of next generation sequencing as an additional diagnostic tool to SMC in ischemic type biliary lesions and/or anastomotic strictures.

Methods

We sequenced the V1-V2 region of the 16S rRNA gene in 242 stored bile samples in patients after LTX and com

SUBMITTER: Grobe B 

PROVIDER: S-EPMC10709207 | biostudies-literature | 2024 Jan-Feb

REPOSITORIES: biostudies-literature