Adjudin improves beta cell maturation, hepatic glucose uptake and glucose homeostasis.

ABSTRACT:

Aims/hypothesis

Recovering functional beta cell mass is a promising approach for future diabetes therapies. The aim of the present study is to investigate the effects of adjudin, a small molecule identified in a beta cell screen using zebrafish, on pancreatic beta cells and diabetes conditions in mice and human spheroids.

Methods

In zebrafish, insulin expression was examined by bioluminescence and quantitative real-time PCR (qPCR), glucose levels were examined by direct measurements and distribution using a fluorescent glucose analogue, and calcium activity in beta cells was analysed by in vivo live imaging. Pancreatic islets of wild-type postnatal day 0 (P0) and 3-month-old (adult) mice, as well as adult db/db mice (i.e. BKS(D)-Lepr^db/JOrlRj), were cultured in vi

SUBMITTER: Ren L 

PROVIDER: S-EPMC10709271 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature