Single-organoid analysis reveals clinically relevant treatment-resistant and invasive subclones in pancreatic cancer.

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal diseases, characterized by a treatment-resistant and invasive nature. In line with these inherent aggressive characteristics, only a subset of patients shows a clinical response to the standard of care therapies, thereby highlighting the need for a more personalized treatment approach. In this study, we comprehensively unraveled the intra-patient response heterogeneity and intrinsic aggressive nature of PDAC on bulk and single-organoid resolution. We leveraged a fully characterized PDAC organoid panel (N = 8) and matched our artificial intelligence-driven, live-cell organoid image analysis with retrospective clinical patient response. In line with the clinical outcomes, we identified patient-specific sensitivities to the sta

SUBMITTER: Le Compte M 

PROVIDER: S-EPMC10709344 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature