Sperm chromatin accessibility's involvement in the intergenerational effects of stress hormone receptor activation.

ABSTRACT: Dexamethasone is a stress hormone receptor agonist used widely in clinics. We and others previously showed that paternal administration of dexamethasone in mice affects the phenotype of their offspring. The substrate of intergenerational transmission of environmentally induced effects often involves changes in sperm RNA, yet other epigenetic modifications in the germline can be affected and are also plausible candidates. First, we tested the involvement of altered sperm RNAs in the transmission of dexamethasone induced phenotypes across generations. We did this by injecting sperm RNA into naïve fertilized oocytes, before performing metabolic and behavioral phenotyping of the offspring. We observed phenotypic changes in discordance with those found in offspring generated by in vitro fertili

SUBMITTER: Fischer V 

PROVIDER: S-EPMC10709351 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature