Serine 937 phosphorylation enhances KCC2 activity and strengthens synaptic inhibition.

ABSTRACT: The potassium chloride cotransporter KCC2 is crucial for Cl^- extrusion from mature neurons and thus key to hyperpolarizing inhibition. Auditory brainstem circuits contain well-understood inhibitory projections and provide a potent model to study the regulation of synaptic inhibition. Two peculiarities of the auditory brainstem are (i) posttranslational activation of KCC2 during development and (ii) extremely negative reversal potentials in specific circuits. To investigate the role of the potent phospho-site serine 937 therein, we generated a KCC2 Thr^934Ala/Ser^937Asp double mutation, in which Ser⁹³⁷ is replaced by aspartate mimicking the phosphorylated state, and the neighbouring Thr⁹³⁴ arrested in the dephosphorylated state. This doub

SUBMITTER: Radulovic T 

PROVIDER: S-EPMC10709408 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature