Ambiguous genes due to aligners and their impact on RNA-seq data analysis.

ABSTRACT: The main scope of the study is ambiguous genes, i.e. genes whose expression is difficult to estimate from the data produced by next-generation sequencing technologies. We focused on the RNA sequencing (RNA-Seq) type of experiment performed on the Illumina platform. It is crucial to identify such genes and understand the cause of their difficulty, as these genes may be involved in some diseases. By giving misleading results, they could contribute to a misunderstanding of the cause of certain diseases, which could lead to inappropriate treatment. We thought that the ambiguous genes would be difficult to map because of their complex structure. So we looked at RNA-seq analysis using different mappers to find genes that would have different measurements from the aligners. We were able to identi

SUBMITTER: Szabelska-Beresewicz A 

PROVIDER: S-EPMC10709571 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature