Validation and calibration of a novel GEM biosensor for specific detection of Cd²⁺, Zn²⁺, and Pb².

ABSTRACT:

Background

In this study, we designed a novel genetic circuit sensitive to Cd²⁺, Zn²⁺ and Pb²⁺ by mimicking the CadA/CadR operon system mediated heavy metal homeostasis mechanism of Pseudomonas aeruginosa. The regular DNA motifs on natural operon were reconfigured and coupled with the enhanced Green Fluorescent Protein (eGFP) reporter to develop a novel basic NOT type logic gate CadA/CadR-eGFP to respond metal ions mentioned above. A Genetically Engineered Microbial (GEM)-based biosensor (E.coli-BL21:pJET1.2-CadA/CadR-eGFP) was developed by cloning the chemically synthesised CadA/CadR-eGFP gene circuit into pJET1.2-plasmid and transforming into Escherichia coli (E. coli)-BL21 bacterial cells.

Results

The GEM-based biosensor cells indicated th

SUBMITTER: Herath HMLPB 

PROVIDER: S-EPMC10709830 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature