MiRNA-ome plasma analysis unveils changes in blood-brain barrier integrity associated with acute liver failure in rats.

ABSTRACT:

Background

Hepatic encephalopathy (HE) symptoms associated with liver insufficiency are linked to the neurotoxic effects of ammonia and other toxic metabolites reaching the brain via the blood-brain barrier (BBB), further aggravated by the inflammatory response. Cumulative evidence documents that the non-coding single-stranded RNAs, micro RNAs (miRs) control the BBB functioning. However, miRs' involvement in BBB breakdown in HE is still underexplored. Here, we hypothesized that in rats with acute liver failure (ALF) or rats subjected to hyperammonemia, altered circulating miRs affect BBB composing proteins.

Methods

Transmission electron microscopy was employed to delineate structural alterations of the BBB in rats with ALF (thioacetamide (TAA) intraperitoneal (ip.) administr

SUBMITTER: Orzel-Gajowik K 

PROVIDER: S-EPMC10709860 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature