DNA polymerases in precise and predictable CRISPR/Cas9-mediated chromosomal rearrangements.

ABSTRACT:

Background

Recent studies have shown that, owning to its cohesive cleavage, Cas9-mediated CRISPR gene editing outcomes at junctions of chromosomal rearrangements or DNA-fragment editing are precise and predictable; however, the underlying mechanisms are poorly understood due to lack of suitable assay system and analysis tool.

Results

Here we developed a customized computer program to take account of staggered or cohesive Cas9 cleavage and to rapidly process large volumes of junctional sequencing reads from chromosomal rearrangements or DNA-fragment editing, including DNA-fragment inversions, duplications, and deletions. We also established a sensitive assay system using HPRT1 and DCK as reporters for cell growth during DNA-fragment editing by Cas9 with dual sgRNAs and found

SUBMITTER: Mehryar MM 

PROVIDER: S-EPMC10709867 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature